A simple radioassay for angiotensin-converting enzyme
Authors:
- J W RyanJRJ W Ryan
- A ChungACA Chung
- C AmmonsCAC Ammons
- M L CarltonMCM L Carlton
- 1 November 1977
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 167 (2) , 501-504
Abstract
Angiotensin-converting enzyme can be measured by the rate of release of 3H-labelled hippurate from p-[3H]benzoylglycylglycylglycine. The product is separable from the substrate by extraction of acidified reaction mixtures with ethyl acetate. Assay results for human serum angiotensin-converting enzyme can be obtained within 1.5 h of receipt of serum samples. Within the limits tested, the assay appears to be specific. However, interference by hitherto unrecognized enzymes of abnormal sera must be ruled out.Keywords
This publication has 17 references indexed in Scilit:
- Design of Specific Inhibitors of Angiotensin-Converting Enzyme: New Class of Orally Active Antihypertensive AgentsScience, 1977
- THE SPECIFICITY AND NATURE OF SERUM‐ANGIOTENSIN‐CONVERTING ENZYME (SERUM ACE) ELEVATIONS IN SARCOIDOSIS*Annals of the New York Academy of Sciences, 1976
- ELEVATION OF ANGIOTENSIN‐CONVERTING ENZYME IN GRANULOMATOUS LYMPH NODES AND SERUM IN SARCOIDOSIS: CLINICAL AND POSSIBLE PATHOGENIC SIGNIFICANCE*Annals of the New York Academy of Sciences, 1976
- A Sensitive Fluorimetric Assay for Serum Angiotensin-con venrting EnzymeAmerican Journal of Clinical Pathology, 1976
- Formation of angiotensin III by angiotensin-converting enzymeBiochemical Journal, 1976
- Kinetic properties of pulmonary angiotensin-converting enzyme. Hydrolysis of hippurylglycylglycineBiochimica et Biophysica Acta (BBA) - Enzymology, 1976
- Synthesis and evaluation of [des-Asp1]angiotensin I as a precursor for [des-Asp1]angiotensin II (angiotensin III)Journal of Medicinal Chemistry, 1975
- Elevation of serum angiotension-converting-enzyme (ACE) level in sarcoidosisThe American Journal of Medicine, 1975
- Studies on the angiotensin converting enzyme with different substratesBiochimica et Biophysica Acta (BBA) - Enzymology, 1970
- THE PREPARATION AND FUNCTION OF THE HYPERTENSIN-CONVERTING ENZYMEJournal of Experimental Medicine, 1956