Biofunctionalization of chalcogenide glass fiber to enhance real time and label free detection by mid infrared spectroscopy

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Abstract
Bio-functionalized chalcogenide infrared optical glass fibers have been designed for evanescent wave mid-infrared spectroscopy. Surface biotinylation of the fiber tapered sensing zone has been achieved by reactivity of a maleimide function on sulfhydryl moieties of the glassy surface. Biotin-streptavidin interactions were studied by fiber evanescent wave spectroscopy. Kinetic measurement comparisons of functionalized and non-functionalized fiber surfaces for various protein concentrations have demonstrated the efficient bio-selectivity of the functionalized glass fibers. The protein enrichment of the functionalized glassy surface allows for a significant increase of the protein detection limit, greater than two orders of magnitude as compared to reference non-functionalized fibers. A detection of minute quantities at concentrations as low as 10 parts-per-billion is demonstrated. This study shows that bio-functionalized chalcogenide optical fibers allow to combine successfully surface bio-selectivity and infrared absorption fingerprints measurements to get a remarkable sensitivity enhancement of fiber evanescent wave detection methods in the mid infrared spectral range.
Funding Information
  • IdEx Bordeaux (GPR Light, GPR Light)
  • Agence Nationale de la Recherche (EQUIPEX+ contract "ANR-21-ESRE-0040", EQUIPEX+ contract "ANR-21-ESRE-0040", EQUIPEX+ contract "ANR-21-ESRE-0040", EQUIPEX+ contract "ANR-21-ESRE-0040", SURF-GLASS-IR Project (ANR-23-CE08-0006-01), SURF-GLASS-IR Project (ANR-23-CE08-0006-01), SURF-GLASS-IR Project (ANR-23-CE08-0006-01))
  • Conseil régional de Bourgogne-Franche-Comté
  • Horizon 2020 Framework Programme (823941 (FUNGLASS))
  • Conseil Régional Aquitaine (Grant 2016-1R10107)

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