Expression and Purification of Soluble Porcine Cystatin 11 in Pichia pastoris
Authors:
- 27 August 2014
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 174 (5) , 1959-1968
Abstract
Cystatin 11 (CST11) belongs to the cystatin type 2 family of cysteine protease inhibitors and exhibits antimicrobial activity in vitro. In this study, we describe the expression and purification of recombinant porcine CST11 in the Pichia pastoris system. We then assess its antimicrobial activity against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Bacillus subtilis by liquid growth inhibition assay. Kinetic studies indicate that the recombinant porcine CST11 has high potency against E. coli and S. aureus. Scanning electronic microscope analysis showed that CST11 might be targeting the bacterial membrane and, thus, could potentially be developed as a therapeutic agent for inhibiting microbe infection without the risk of antibiotic resistance.Keywords
This publication has 11 references indexed in Scilit:
- Molecular Cloning, Recombinant Expression, and Antimicrobial Activity of EC-hepcidin3, a New Four-Cysteine Hepcidin Isoform fromEpinephelus coioidesBioscience, Biotechnology, and Biochemistry, 2013
- Cystatins in Immune SystemJournal of Cancer, 2013
- Antimicrobial Activity and Molecular Mechanism of the CRES ProteinPLOS ONE, 2012
- Overview on the recent study of antimicrobial peptides: Origins, functions, relative mechanisms and applicationPeptides, 2012
- Expression and purification of soluble porcine CTLA-4 in yeast Pichia pastorisProtein Expression and Purification, 2012
- Membrane active antitumor activity of NK-18, a mammalian NK-lysin-derived cationic antimicrobial peptideBiochimie, 2012
- Soluble expression and purification of a crab antimicrobial peptide scygonadin in different expression plasmids and analysis of its antimicrobial activityProtein Expression and Purification, 2010
- Towards novel anti-cancer strategies based on cystatin functionCancer Letters, 2006
- Immunolocalization and Regulation of Cystatin 12 in Mouse Testis and Epididymis1Biology of Reproduction, 2005
- Cystatin 11: A New Member of the Cystatin Type 2 FamilyEndocrinology, 2002