Isolation of Highly Active Photosystem II Particles from a Mutant of Chlamydomonas reinhardtii

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Abstract
Highly active photosystem‐II particles were rapidly isolated using detergents and obtained in good yield from a mutant of the green alga Chlamydomonas reinhardtii. The particles are completely devoid of reaction centers of photosystem I, and of the secondary electron acceptor to photosystem II. They show: (a) a specific activity (ΔA of C550/unit chlorophyll) 4–7‐times that of the starting material and of spinach chloroplasts; (b) an antenna size of 40 to 50 chlorophyll molecules containing little light‐harvesting chlorophyll a/b complex (chlorophyll a/chlorophyll b= 4–6.4); (c) a ratio of variable to dark‐adapted fluorescence yield of up to 3.Further treatment of these particles by ion‐exchange chromatography largely removes five proteins and further decreases the antenna size with little loss in primary photoactivity.

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