A capillary electrophoresis mobility shift assay for protein-DNA binding affinities free in solution
Open Access
- 15 September 1998
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 26 (18) , 4304-4305
Abstract
Quantitative determination of dissociation constants for DNA-protein complexes will help clarify the molecular mechanisms of transcription, replication and DNA repair. A practical capillary electrophoresis mobility shift assay (CEMSA) for protein—DNA affinities free in solution is presented. The method is fast and simple, precise and general. The speed (Kd = 35 ± 4 nM) to demonstrate the utility of the method.Keywords
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