Caspase activation in response to cytotoxic Rana catesbeiana ribonuclease in MCF‐7 cells

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Abstract
Rana catesbeiana ribonuclease (RC‐RNase) and onconase were proven to own anti‐tumor activity. While molecular determinants of onconase‐induced cell death have become more explicit, the RC‐RNase‐induced death pathway remains presently unknown. Here we demonstrated that RC‐RNase‐induced molecular cascades in caspase‐3‐deficient MCF‐7 cells did not include activation of initiation caspase‐8 and ‐9. Cleavage timing suggested that procaspase‐2 and ‐6 might be processed by active caspase‐7 in MCF‐7 cells. Caspase‐7 was also responsible for cleavage of the poly(ADP‐ribose) polymerase. Furthermore, we reported that overexpression of Bcl‐XL could raise the survival rates of MCF‐7 cells treated with RC‐RNase and onconase.

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