Reconstitution of lamin assembly on nuclear pore complex-containing membranes

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Abstract
Intermediate filaments called lamins line the metazoan nuclear envelope and organize the nucleus and genome. Unlike actin and microtubules, purified intermediate filament proteins assemble into non-physiological structures, making it difficult to connect lamin functions to their assembly and regulation. To overcome this challenge and shed light on physiological lamin assembly mechanisms, we conducted biochemical studies of lamin-B3 endogenously present in Xenopus laevis egg extract, which recapitulates physiological context. When we mimicked nucleoplasm conditions that would support assembly of lamin filaments in intact cells, lamin-B3 assembled into higher-order structures resembling filamentous meshworks without accompanying nuclear assembly. This ectopic lamin assembly occurs on nuclear pore complex-containing membranes, but does not apparently recruit known nuclear lamina components, demonstrating that a lamin assembly process is partially separable from the rest of the nuclear lamina and nucleus. This assembly assay in the physiological context of cellular components opens the door to further dissecting nuclear lamina function in nuclear organization.
Funding Information
  • National Institutes of Health (F32GM142145)
  • National Institutes of Health (DP2GM150017)
  • National Institutes of Health (R01GM130134)
  • National Institutes of Health (R01GM106023)
  • National Institutes of Health (R01GM157589)
  • National Science Foundation (NSF CAREER Award 2341058)

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