Molecular cloning of UBE2G, encoding a human skeletal muscle-specific ubiquitin-conjugating enzyme homologous to UBC7 of C. elegans

Authors:
  • AKA. Kawai
    A. Kawai
  • TFT. Fujiwara
    T. Fujiwara
  • HMH. Maekawa
    H. Maekawa
  • YHY. Hirai
    Y. Hirai
  • YNY. Nakamura
    Y. Nakamura
  • ETE. Takahashi
    E. Takahashi
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Abstract
From a human fetal-brain cDNA library we isolated a novel ubiquitin-conjugating enzyme. The cDNA, designated UBE2G, contained an open reading frame of 510 nucleo-tides encoding 170 amino acids. The predicted peptide product showed 74% identity at the amino acid level with UBC7 of C. elegΑns, a high degree of homology with UBC7s of other species, and significant homologies with other subgroups of UBCs. Northern-blot analysis revealed strong expression of 4.4-kb, 2.4-kb, and 1.6-kb transcripts in skeletal muscle. Weak expression was observed in 15 other tissues examined. Radiation-hybrid mapping localized this cDNA to chromosome band lq42.