Pharmacokinetics of niazirin from Moringa oleifera Lam in rats by UPLC‐MS/MS: Absolute bioavailability and dose proportionality
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Open Access
Abstract
Niazirin is a nitrile glycoside isolated from the pods and leaves of Moringa oleifera and possesses antidiabetic activity. However, its pharmacokinetic properties have not been well studied yet. The present study aims to establish a specific, rapid, and simple method for the determination of niazirin in rat plasma and apply it to evaluate its absolute bioavailability and dose proportionality. MS/MS detection was performed in multiple reaction monitoring modes with negative electrospray ionization. The precursor–product ion transitions were m/z 278.1 [M‐H]− → 130.8 for niazirin and m/z 316.9 [M‐H]− → 271.0 for IS. The intra‐ and inter‐day precision (RSD) was 0−∞ and Cmax at the three doses were y = 177.39x + 99.716 (r2 = 0.9962), and y = 63.152x + 19.531 (r2 = 0.9961), respectively. The study provides a scientific basis for safety evaluation and new clinical research on niazirin.Keywords
Funding Information
- National Natural Science Foundation of China (32172139, 32172203, 81903940)
This publication has 21 references indexed in Scilit:
- The molecular mechanism underlying the hepatoprotective potential of Moringa oleifera leaves extract against acetaminophen induced hepatotoxicity in miceJournal of Functional Foods, 2015
- Quantitative analysis of the nutritional components in leaves and seeds of the Persian Moringa peregrina (Forssk.) FioriPharmacognosy Research, 2015
- Antioxidant, Antimicrobial and Phytochemical Variations in Thirteen Moringa oleifera Lam. CultivarsMolecules, 2014
- Soluble Extract from Moringa oleifera Leaves with a New Anticancer ActivityPLOS ONE, 2014
- Preparative Isolation of Bioactive Nitrile Glycoside “Niazirin” from the Fruits of Moringa oleifera Using Fast Centrifugal Partition ChromatographySeparation Science and Technology, 2011
- Potential anti-inflammatory phenolic glycosides from the medicinal plant Moringa oleifera fruitsBioorganic & Medicinal Chemistry, 2010
- Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in ratsFood and Chemical Toxicology, 2010
- In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leavesFood and Chemical Toxicology, 2009
- Determination of bioactive nitrile glycoside(s) in drumstick (Moringa oleifera) by reverse phase HPLCFood Chemistry, 2007
- Isolation and Structure Elucidation of New Nitrile and Mustard Oil Glycosides from Moringa oleifera and Their Effect on Blood PressureJournal of Natural Products, 1994