Aging of Wound Healing in an Experimental Model in Mice

Authors:
  • AHAbraham Hirshberg
    Abraham Hirshberg
  • DDDan Fundoiano Dayan
    Dan Fundoiano Dayan
  • JBJose J. Bubis
    Jose J. Bubis
  • MWMoshe Wolman
    Moshe Wolman
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Abstract
Healing of incised wounds in the backs of mice was studied to establish criteria that would allow the timing of stages during cutaneous wound healing. Starting from day 4 after the incisions were made, three mice were killed every 3 days until day 40. Sections from wounds were stained with hematoxylin-eosin, a reticulin procedure, and picrosirius red. Unstained sections were used for blue autofluorescence. The significance of the findings from the ultraviolet study was limited, but the picrosirius red-polarized light procedure was found to be useful for estimating the age of the repair process as polarization colors of greater wavelengths progressively increased with the age of the lesion.