Supplementary Figure 2 from Tumor Homing Nanoparticle Immunotherapy for Acute Clearance and Mitigation of T-cell Exhaustion in Humanized High-Risk Neuroblastoma

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Abstract
Supplementary Figure 2: NP uptake, endothelial cell and macrophage proliferation, cytokine production, and immune cell proliferation. (A-C) Flow cytometry analysis of NP uptake by CD31+ endothelial cells, CD62E+CD31+ endothelial cells, and CD33+ macrophages in the primary tumor (A), metastatic liver (B), and metastatic lungs (C) (N=6 mice per group). (D-F) Proliferation of CD31+ endothelial cells, CD62E+ cells, and CD33+ macrophages in the primary tumor (D), metastatic liver (E), and metastatic lungs (F) (N=6 mice per group). (G-H) Flow cytometry analysis of expression of the indicated cytokine by CD33+ macrophages in the primary tumor (G), metastatic liver (H), and metastatic lungs (I) (N=6 mice per group). (J) Flow cytometry analysis of NP uptake by CD45+ leukocytes, CD11c+ DCs, and CD56+ NK cells in the blood (N=6 mice per group). (K) Proliferation of CD45+ leukocytes, CD11c+ DCs, and CD56+ NK cells in the blood (N=6 mice per group). For flow cytometry, technical duplicates were averaged and plotted. Error bars, ± SEM. P values were calculated using 2-way ANOVA. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05, n.s, not significant.