Preliminary study of HPV integration status on the occurrence and development of Vaginal intraepithelial neoplasia

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Abstract
Vaginal intraepithelial neoplasm (VaIN), a precancerous lesion of vaginal cancer, increased distinctly in the past decade, while its occurrence after performing hysterectomy came to attention nowadays. As hr-HPV DNA integrates into host chromosome, transient HPV infection could transform into persistent infection to trigger high-grade intraepithelial neoplasm or HPV-related cancer. High-throughput Viral Integration Detection (HIVID) is a novel genetic sequencing method detecting HPV integration breakpoints for more precise sequence. There is no systematic study on the mechanism and risk assessment prediction system of VaIN in the vaginal stump after total hysterectomy for CIN. In this study, 24 cases suffered from VaIN were involved. HPV integration was detected in 11 cases, furthermore, we discovered HPV 16 and 73, chromosome 1 and 2 possessed most HPV integration sites while EMBP1, CLO5A1, EHF, ELF5 as dominate hot spots. Taken clinical outcome into account, we found a significant difference between HPV integration occurrence and VaIN(p = 0.011). HPV integration could serve as a predictive marker for monitoring and management of VaIN in a way.

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