Dopaminergic neurons linking threat processing to cardiac modulation and locomotor responses

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Abstract
Heartbeat and behavior are tightly coordinated during defensive states, yet the neuronal mechanisms linking threat processing to cardiac modulation—and the potential contribution of cardiac dynamics to behavioral output—remain poorly understood. Here, using simultaneous recordings of heartbeat and behavior in tethered Drosophila , we show that mechanical threat triggers locomotion together with rapid cardiac deceleration. We identify two pairs of dopaminergic neurons, termed DA-WED neurons, that mediate this cardiac response. Silencing these neurons markedly attenuates threat-induced cardiac deceleration, whereas optogenetic activation induces cardiac deceleration in the absence of threat. Two-photon calcium imaging further shows that DA-WED neurons are activated by mechanical threat, and manipulating their activity bidirectionally modulates threat-induced locomotion. Linking cardiac dynamics to behavior, direct optogenetic manipulation of cardiomyocytes that quantitatively reproduces threat-evoked cardiac deceleration is accompanied by increased locomotion. Together, these results identify a dopaminergic descending pathway that links threat processing to cardiac modulation and suggest that cardiac dynamics may contribute to shaping defensive behavior.
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Funding Information
  • Japan Society for the Promotion of Science (KAKENHI 24K18157)
  • Japan Science and Technology Agency (10.52926/jpmjax242d)
  • Yamaguchi Educational and Scholarship Foundation
  • Japan Agency for Medical Research and Development (JP21gm310010)
  • Takeda Science Foundation
  • Torray Foundation
  • Naito Foundation
  • WPI-IRCN
  • Japan Society for the Promotion of Science (KAKENHI 26K18425)

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