Comparison of multiple genes of spring viremia of carp viruses isolated in the United States
- 18 October 2006
- journal article
- research article
- Published by Springer Nature in Virus Genes
- Vol. 35 (1) , 87-95
Abstract
Five spring viremia of carp viruses (SVCV), Rhabdovirus carpio, were isolated in the United States (US) between 2002 and 2004. Single tube reverse transcription-polymerase chain reaction (RT-PCR) was used to generate overlapping cDNA fragments from the US isolates of SVCV. Multiple pairs of specific primers were designed to amplify a portion of the phosphoprotein gene, the matrix gene, and the glycoprotein gene of SVCV genogroup Id (corresponding to nucleotides 2174–4942 of GenBank accession NC_002803). Sequences were proofread and aligned to generate a consensus sequence for each isolate. Phylogenetic analysis of the 2705 nucleotide consensus sequence revealed that all five US isolates belong to SVCV genogroup Ia, Asian origin isolates, and a PCR primer binding site unique to SVCV genogroup Ia was identified.Keywords
This publication has 32 references indexed in Scilit:
- First Report of Spring Viremia of Carp Virus (SVCV) in Wild Common Carp in North AmericaJournal of Aquatic Animal Health, 2004
- Nucleotide sequence analysis of the glycoprotein gene of putative spring viraemia of carp virus and pike fry rhabdovirus isolates reveals four genogroupsDiseases of Aquatic Organisms, 2003
- First Report of Spring Viremia of Carp Virus (SVCV) in North AmericaJournal of Aquatic Animal Health, 2002
- The glycoprotein genes and gene junctions of the fish rhabdoviruses spring viremia of carp virus and hirame rhabdovirus: analysis of relationships with other rhabdovirusesVirus Research, 1996
- Enzyme‐linked immunosorbent assay (ELISA) for the detection of spring viraemia of carp virus (SVCV) in tissue homogenates of the carp, Cyprinus carpio L.Journal of Fish Diseases, 1993
- Rapid detection of SVC virus antigen in infected cell cultures and clinically diseased carp by the enzyme-linked immunosorbent assay (ELISA)Journal of Applied Ichthyology, 1991
- Rapid detection of fish rhabdoviruses by the enzyme-linked immunosorbent assay (ELISA)Aquaculture, 1984
- Spring viraemia of carp virus (SVCV): comparison of immunoperoxidase, fluorescent antibody and cell culture isolation techniques for detection of antigenJournal of Fish Diseases, 1984
- Quantitativer Nachweis des Spring Viremia of Carp Virus (SVCV)‐Antigens mit Hilfe der Immunofluoreszenz‐und ImmunoperoxidaseplaquestechnikZentralblatt für Veterinärmedizin Reihe B, 1983
- Structural proteins of spring viremia virus of carpBiochemical and Biophysical Research Communications, 1973