Stimulus interval, rate and direction differentially regulate phosphorylation for mechanotransduction in neonatal cardiac myocytes
- 8 August 2007
- journal article
- Published by Wiley in FEBS Letters
- Vol. 581 (22) , 4241-4247
Abstract
The effect of interval, direction and rate of strain on mechanotransduction in neonatal rat cardiomyocytes is determined for focal adhesion kinase (Y397pFAK), extracellular signal‐regulated kinase ERK1/2 (Thr183/Tyr185) and paxillin (pY31) and phosphorylation time courses to 10% strain assessed. Cells are non‐responsive at 5 min but recover at 15 min (P < 0.03) with FAK nuclear translocation by 30 min. Cyclic biaxial strain increased phosphorylation from slower to faster rates (P < 0.05). Uniaxial strain to groove‐aligned myocytes increased FAK and ERK1/2 phosphorylation transversely more than longitudinally (P < 0.05). Mechanotransduction may have a refractory period of 5 min and differentiate directions and rates of strain.Keywords
This publication has 22 references indexed in Scilit:
- Selective adapter recruitment and differential signaling networks by VEGF vs. shear stressProceedings of the National Academy of Sciences, 2007
- Altered PKC expression and phosphorylation in response to the nature, direction, and magnitude of mechanical stretchCanadian Journal of Physiology and Pharmacology, 2007
- Cardiac dysfunction and heart failure are associated with abnormalities in the subcellular distribution and amounts of oligomeric muscle LIM proteinAmerican Journal of Physiology-Heart and Circulatory Physiology, 2007
- Anisotropic mechanosensing by mesenchymal stem cellsProceedings of the National Academy of Sciences, 2006
- Focal Adhesion Kinase Is Activated and Mediates the Early Hypertrophic Response to Stretch in Cardiac MyocytesCirculation Research, 2003
- Inhibition of fibroblast proliferation in cardiac myocyte cultures by surface microtopographyAmerican Journal of Physiology-Cell Physiology, 2003
- Streptomycin and intracellular calcium modulate the response of single guinea‐pig ventricular myocytes to axial stretchThe Journal of Physiology, 2003
- Anisotropic stretch‐induced hypertrophy in neonatal ventricular myocytes micropatterned on deformable elastomersBiotechnology and Bioengineering, 2002
- Distinct Signaling Pathways Are Activated in Response to Mechanical Stress Applied Axially and Transversely to Skeletal Muscle FibersJournal of Biological Chemistry, 2002
- Regulation of Cardiac Myocyte Protein Turnover and Myofibrillar Structure In Vitro by Specific Directions of StretchCirculation Research, 1999