Nonendothelial‐derived nitric oxide activates the ATP‐sensitive K+ channel of vascular smooth muscle cells
Authors:
- Yutaka NakayaYNYutaka Nakaya
- Hideki MoritokiHMHideki Moritoki
- 23 May 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 345 (1) , 47-49
Abstract
To determine whether endogenous nitric oxide (NO) opens the ATP‐sensitive K+ channel (KATP channel), we investigated the effect of nonendothelial‐derived NO on this channel in cultured smooth muscle cells of the porcine coronary artery by the patch‐clamp technique. In the cells pretreated with endotoxin, the addition of 10−4 M l‐arginine generated NO and activated the KATP channel. Activation of this channel was suppressed by pretreatment with 10−3 M N G‐methyl‐l‐arginine or 10−3 M N x‐nitro‐l‐arginine methyl ester, each of which is a specific antagonist of the l‐arginine‐NO pathway, and by 10−6 M Methylene blue, which blocks guanylate cyclase. The activation of the KATP channel by l‐arginine‐NO pathway is expected to produce hyperpolarization of the cell membrane and relaxation of vascular smooth muscle cells.Keywords
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