Simple Amplification‐Based Assay: A Nucleic Acid–Based Point‐of‐Care Platform for HIV‐1 Testing

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Abstract
Background. A new nucleic acid-based assay (simple amplification-based assay [SAMBA]) for rapid visual detection of human immunodeficiency virus-type 1 (HIV-1) by dipstick is described. The assay was designed to be simple, stable, robust, self-contained, and capable of detecting a broad spectrum of HIV-1 subtypes and recombinant forms.

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