High-throughput, low-loss, low-cost, and label-free cell separation using electrophysiology-activated cell enrichment

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Abstract
Significance: Cell separation is a fundamental process in biomedicine, but is presently complicated, cumbersome, and expensive. We present a technique that can sort cells at a rate equivalent to or faster than gold-standard techniques such as fluorescence- and magnetic-activated cell sorting, but can do it label-free and with very low cell loss. The system uses dielectrophoresis to sort cells electrostatically, using an electrode chip that eschews microfabrication in favor of a laminate drilled with 397 electrode-bearing wells. This high level of parallelization makes the system immune to the bubbles that limit labs-on-chip, while also increasing capacity and throughput to levels comparable with high-throughput FACS and MACS, whereas the chip is cheap enough to be disposable, preventing interseparation contamination.

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