SHORT COMMUNICATION:Response of nicotianamine synthase activity to Fe-deficiency in tobacco plants as compared with barley

Authors:
  • KHKiyoko Higuchi
    Kiyoko Higuchi
  • NNNaoko-Kishi Nishizawa
    Naoko-Kishi Nishizawa
  • HYHirotaka Yamaguchi
    Hirotaka Yamaguchi
  • VRVolker Römheld
    Volker Römheld
  • HMHorst Marschner
    Horst Marschner
  • SMSatoshi Mori
    Satoshi Mori
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Abstract
The duckweeds Lemna gibba L. and Lemna minor L. only grew well in undisturbed culture under axenic conditions in low light intensity when provided with a suitable energy source such as glucose. In media containing N03-N gibbosity (a convex ventral surface) was induced in the presence of the chelating agent ethylene-diamine-di-o-hydroxyphenylacetic acid (EDDHA). In nutrient solutions containing NO3-N as the only N source, but without EDDHA, L. gibba occasionally exhibited gibbosity in culture solutions of 40 cm3 volumes. More fronds were induced to exhibit gibbosity when the volume of the culture medium was increased from 40 cm3 to 200 cm3. Gibbosity was never induced in L. minor, neither was it induced in L. gibba in media containing NH4-N, even in the presence of NO3-N. There was no direct correlation between the occurrence of gibbosity and frond growth rate, but gibbosity occurred only when there was good frond growth.