Development of a Standardized Susceptibility Test for Campylobacter with Quality-Control Ranges for Ciprofloxacin, Doxycycline, Erythromycin, Gentamicin, and Meropenem
- 1 June 2004
- journal article
- research article
- Published by SAGE Publications in Microbial Drug Resistance
- Vol. 10 (2) , 124-131
Abstract
A standardized agar dilution susceptibility testing method was developed for Campylobacter that consisted of testing on Mueller–Hinton medium supplemented with 5% defibrinated sheep blood in an atmosphere of 10% CO 2 , 5% O 2 , and 85% N 2 . Campylobacter jejuni ATCC 33560 was identified as a quality-control (QC) strain. Minimal inhibitory concentration (MIC) QC ranges were determined for two incubation time/temperature combinations: 36°C for 48 hr and 42°C for 24 hr. Quality-control ranges were determined for ciprofloxacin, doxycycline, erythromycin, gentamicin, and meropenem. For all antimicrobial agents tested at both temperatures, 95–100% of the QC MIC results fell within recommended QC ranges. Twenty-one Campylobacter clinical isolates, encompassing five species of Campylobacter ( C. jejuni , C. coli , C. jejuni , subsp. doylei , C. fetus , and C. lari ) were tested in conjunction with the C. jejuni QC strain. While C. jejuni and C. coli could be reliably tested under both test conditions, growth of C. jejuni subsp. doylei , C. fetus , and C. lari isolates was inconsistent when incubated at 42°C. Therefore, it is recommended that these species only be tested at 36°C.Keywords
This publication has 15 references indexed in Scilit:
- Comité de l'Antibiogramme de la Société Française de Microbiologie Report 20031,21This publication is protected by copyright. No part or translation of it may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Chairholder of Comité de l'Antibiogramme de la Société Française de Microbiologie, Centre Hospitalier Universitaire Henri Mondor, Service de Bactériologie-Virologie-Hygiène, 51, ave du Mal de Lattre de, Tassigny, 94010 Créteil Cedex, France. The Comité de l'Antibiogramme hereby grants permission to reproduce this publication for use in laboratory procedure manuals at a single site, and limited portions of this publication for scientific purposes.2Members (2002): G. Carret, J.D. Cavallo, H. Chardon, C. Chidiac, P. Choutet, P. Courvalin, H. Dabernat, H. Drugeon, L. Dubreuil, F. Goldstein, V. Jarlier, R. Leclercq, M.H. Nicolas-Chanoine, A. Philippon, C. Quentin-Noury, B. Rouveix, J. Sirot, C.J. Soussy.International Journal of Antimicrobial Agents, 2003
- Antimicrobial susceptibility testing of Campylobacter jejuni: a comparison between Etest and agar dilution methodDiagnostic Microbiology and Infectious Disease, 2003
- Comparison of the Etest and agar dilution for in vitro antimicrobial susceptibility testing of CampylobacterJournal of Antimicrobial Chemotherapy, 2002
- Diversity of Campylobacter Isolates from Retail Poultry Carcasses and from Humans as Demonstrated by Pulsed-Field Gel ElectrophoresisJournal of Food Protection, 2002
- Prevalence of Salmonella and Campylobacter in retail chicken meat in SpainInternational Journal of Food Microbiology, 2002
- Incidence of Campylobacter and Salmonella isolated from retail chicken and associated packaging in South WalesLetters in Applied Microbiology, 2001
- Prevalence ofCampylobacterspp., Escherichia coli, andSalmonellaSerovars in Retail Chicken, Turkey, Pork, and Beef from the Greater Washington, D.C., AreaApplied and Environmental Microbiology, 2001
- Comparison of two agar dilution methods and three agar diffusion methods, including the Etest, for antibiotic susceptibility testing of thermophilic Campylobacter speciesClinical Microbiology and Infection, 1999
- Quinolone-ResistantCampylobacter jejuniInfections in Minnesota, 1992–1998New England Journal of Medicine, 1999
- Quinolone resistance in campylobacter isolated from man and poultry following the introduction of fluoroquinolones in veterinary medicineJournal of Antimicrobial Chemotherapy, 1991