Regular transcutaneous myostimulation alters skeletal muscle phospholipid fatty acid composition and oxidative stability in rabbits

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Abstract
This experiment was performed to study the effects of regular, isometric, electrically induced \ud contractions on skeletal muscle phospholipid (PL) and triglyceride (TG) fatty acid profile and oxidative \ud stability. Transcutaneous electrical myostimulation of \ud lo ngissimus dorsi\ud (30\ud Hz, 20\ud ms impulse length, \ud 10 mA, for 2×20\ud min daily) was performed on 8 Pannon White male rabbits for 4 weeks; 8 animals \ud were set as control. Muscle TG and PL fatty acid profiles were determined by capillary gas \ud chromatography after lip\ud id fractionation. In the PL, the proportion of pentadecanoic (C15:0), palmitic \ud (C16:0), linoleic (C18:2 n-6) and γ-linolenic (C18:3 n-6) acids decreased significantly compared to the \ud control, as well as the sum of total saturated fatty acids. Increasing pr\ud oportions of palmitoleic (C16:1n-7), gondoic (C20:1 n-9), arachidonic (C20:4 n-6), eicosapentaenoic (C20:5 n-3) and docosahexaenoic \ud (C22:6 n-3) acids were measured. The C20:4 n6/C20:3 n-6 ratio (estimated ∆5 desaturase activity), the \ud sum of polyunsaturat\ud ed fatty acids (PUFA) of 20 to 22 carbon chain length, the sum of total n-6 fatty acids and the unsaturation index increased. In the TG, stearic acid (C18:0) proportion was significantly \ud decreased by the stimulation. Electrical treatment increased oxidative stability as assessed by \ud thiobarbituric acid reactive substances (TBARS)

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