Chronically implantable μLED arrays for optogenetic cortical surface stimulation in mice

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Abstract
Cortical implants are a proven clinical neurotechnology with the potential to transform our understanding of cognitive processes. These processes rely on complex neuronal networks that are difficult to selectively probe or stimulate. Optogenetics offers cell-type specificity, but achieving the density and coverage required for chronic, high-resolution modulation remains a challenge. We fabricated 100-element μ LED arrays (200 μ m pixel pitch, 2 × 2 mm 2 footprint) coupled into chronically implantable systems for optogenetic stimulation of the mouse cortex. The μ LEDs remain stable for over 300 hours continuous operation time in vivo, allowing for months-long chronic experiments. Simultaneous electrophysiology recordings confirmed robust neuronal responses at low μ LED drive currents (< 5 mA), minimising thermal effects. Here we show that our device can be chronically implanted in freely-behaving mice and drive behavioural outcomes from spatiotemporal, patterned, optogenetic stimulation of auditory cortical circuits.
Funding Information
  • EC | Horizon 2020 Framework Programme (964568)
  • EC | Horizon 2020 Framework Programme (964568)
  • EC | Horizon 2020 Framework Programme (964568)
  • Royal Academy of Engineering

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