Photothermal-Responsive Conjugated Polymer Nanoparticles Triggered Precise Augmentation of Microalgal Photosynthesis

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Abstract
Exploring precise temperature-based photosynthesis regulation strategies will afford promising opportunities for controllable and efficient enhancement of photosynthesis. In this work, we developed a type of photothermal conjugated polymer nanoparticles (T-NPs) based on TZQ-TDPP (poly[2,5-bis(2-ethylhexyl)-3,6-di(thiophen-2-yl)-2,5-dihydropyrrolo[3,4-c]pyrrole-1,4-dione-co-(6,7-bis(4-((2-decyltetradecyl)oxy)phenyl) -[1,2,5] thiadiazolo[3,4-g]quinoxaline)]). T-NPs possess the desired photothermal conversion performance, with their photothermal conversion efficiency reaching up to 80.52%. The introduction of T-NPs enables the controllable regulation of the microenvironment temperature of Chlorella pyrenoidosa (C. pyre), thereby achieving accurate regulation on its photosynthetic activity. Under optimal conditions, T-NPs accelerate light-dependent reactions, leading to increased oxygen evolution and NADP+, NADPH, and ATP contents up to 46%, 20%, 23%, and 103%, respectively. Moreover, the Rubisco activity of C. pyre under light-independent reactions increased by 258%. The chlorophyll fluorimeter parameters and chlorophyll fluorescence kinetics curves also exhibited varying degrees of increase, demonstrating that the introduction of T-NPs improves the photosystem II (PSII)-related process and enhances the photosynthesis process of C. pyre. Integrated proteomic profiling revealed the regulatory mechanism of T-NPs on the photosynthesis of C. pyre and confirmed the upregulation of the Rubisco activity in C. pyre. This study provides a strategy for precise regulation of photosynthesis through photothermal-responsive conjugated polymer nanoparticles, advancing the development of photosynthetic organism-based sustainable energy and biomanufacturing.
Funding Information
  • National Key Research and Development Program of China (2023YFC3404200, 2023YFC3404202, 2023YFE0105200)
  • K. C. Wong Education Foundation (GJTD-2020-02)
  • Natural Science Foundation of Beijing Municipality (Z220025)
  • National Natural Science Foundation of China (22020102005, 22021002, 22307118, 22322507)
  • Chinese Academy of Sciences (YSBR-050)

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