Human recombinant interleukin 1 stimulates collagenase and prostaglandin E2 production by human synovial cells.
Authors:
- J M DayerJDJ M Dayer
- B de RochemonteixBRB de Rochemonteix
- B BurrusBBB Burrus
- S DemczukSDS Demczuk
- C A DinarelloCDC A Dinarello
Open Access
- 1 February 1986
- journal article
- research article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 77 (2) , 645-648
Abstract
The pathogenesis and progression of rheumatoid arthritis involves the production of biologically active lymphokines and monokines. Of these, interleukin 1 (IL-1) has been somewhat of a controversial molecule because it seems to evoke various biological responses in several different tissues. In these studies we demonstrate that three biological properties of human monocyte-derived IL-1 (T-lymphocyte activation and human synovial cell prostaglandin E2 and collagenase production) co-purify. The complementary DNA for the prominent pI 7 form of human IL-1 was expressed, purified, and tested. Any controversy now appears resolved since homogeneous recombinant human IL-1 stimulates prostaglandin E2 and collagenase from human synovial cells as well as activates T cells in vitro.Keywords
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