Inhibition of the oxygen-evolving complex of photosystem II and depletion of extrinsic polypeptides by nickel
- 23 June 2007
- journal article
- Published by Springer Nature in BioMetals
- Vol. 20 (6) , 879-889
Abstract
The toxic effect of Ni2+ on photosynthetic electron transport was studied in a photosystem II submembrane fraction. It was shown that Ni2+ strongly inhibits oxygen evolution in the millimolar range of concentration. The inhibition was insensitive to NaCl but significantly decreased in the presence of CaCl2. Maximal chlorophyll fluorescence, together with variable fluorescence, maximal quantum yield of photosystem II, and flash-induced fluorescence decays were all significantly declined by Ni2+. Further, the extrinsic polypeptides of 16 and 24 kDa associated with the oxygen-evolving complex of photosystem II were depleted following Ni2+ treatment. It was deduced that interaction of Ni2+ with these polypeptides caused a conformational change that induced their release together with Ca2+ from the oxygen-evolving complex of photosystem II with consequent inhibition of the electron transport activity.Keywords
This publication has 47 references indexed in Scilit:
- Copper effect on the protein composition of photosystem IIPhysiologia Plantarum, 2000
- Modulation of Flash-Induced Photosystem II Fluorescence by Events Occurring at the Water Oxidizing ComplexBiochemistry, 1999
- Characteristic changes of function and structure of Photosystem II during strong-light photoinhibition under aerobic conditionsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1995
- The action of mercury on the binding of the extrinsic polypeptides associated with the water oxidizing complex of photosystem IIFEBS Letters, 1995
- Molecular mechanism of action of Pb2+ and Zn2+ on water oxidizing complex of photosystem IIFEBS Letters, 1994
- Calcium activation of photosynthetic water oxidationBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1991
- The release of polypeptides and manganese from oxygen‐evolving photosystem II preparations following zinc‐treatmentFEBS Letters, 1985
- Water‐soluble 17 and 23 kDa polypeptides restore oxygen evolution activity by creating a high‐affinity binding site for Ca2+ on the oxidizing side of Photosystem IIFEBS Letters, 1984
- Photosynthetic oxygen evolution does not require the participation of polypeptides of 16 and 24 kilodaltonsBiochemical and Biophysical Research Communications, 1984
- A highly resolved, oxygen‐evolving photosystem II preparation from spinach thylakoid membranesFEBS Letters, 1981