Efficient enzymatic synthesis of L ‐ascorbyl palmitate using Candida antarctica lipase B‐embedded metal‐organic framework
- 2 October 2021
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 38 (1) , e3218
Abstract
The Candida antarctica lipase B (CALB) was embedded in the metal‐organic framework, zeolitic imidazolate framework‐8 (ZIF‐8), and applied in the enzymatic synthesis of L ‐ascorbic acid palmitate (ASP) for the first time. The obtained CALB@ZIF‐8 achieved the enzyme loading of 80 mg g −1 with 11.3 U g −1 (dry weight) unit activity, 59.8% activity recovery, and 92.7% immobilization yield. Under the optimal condition, ASP was synthesized with over 75.9% conversion of L ‐ascorbic acid in a 10‐batch reaction. Continuous synthesis of ASP was subsequently performed in a packed bed bioreactor with an outstanding average space–time yield of 58.1 g L −1 h −1 , which was higher than ever reported continuous ASP biosynthesis reactions.Keywords
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