Sequence Studies of Poliovirus RNA. IV. Nucleotide Sequence Complexities of Poliovirus Type 1, Type 2 and Two Type 1 Defective Interfering Particles RNAs, and Fingerprint of the Poliovirus Type 3 Genome

Authors:
  • YLYuan Fon Lee
    Yuan Fon Lee
  • NKNaomi Kitamura
    Naomi Kitamura
  • ANAkio Nomoto
    Akio Nomoto
  • EWEckard Wimmer
    Eckard Wimmer
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Abstract
The 32P-labelled genomes of poliovirus type 1, 2 and 3 have been digested with RNase T1 and the products separated by two-dimensional gel electrophoresis. All three fingerprints differ in the separation pattern of the large oligonucleotides. The molar yields of the large RNase T1-resistant oligonucleotides of type 1 and type 2 RNA of poliovirus RNA are close to one. By comparing the yields of these oligonucleotides to the amount of RNA from which they originated, the chain length of type 1 poliovirus RNA was found to be 7851±567 nucleotides (mol. wt. 2.66±0.19 × 106) and that of poliovirus type 2, 8181±578 nucleotides (mol. wt. 2.77±0.19 × 106). The chain length of two defective interfering particle (DI) RNAs of poliovirus type 1 were determined to be 7042±999 nucleotides for DI(1) and 6639±674 nucleotides for DI(2).