Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays
Open Access
- 1 June 2002
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (6) , 3169-3171
Abstract
A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by melting point curve analysis.Keywords
This publication has 8 references indexed in Scilit:
- Use of Real-Time Polymerase Chain Reaction and Molecular Beacons for the Detection of Escherichia coli O157:H7Analytical Biochemistry, 2001
- Molecular Beacon Polymerase Chain Reaction Detection of Escherichia coli O157:H7 in MilkJournal of Food Protection, 2000
- Semi-automated fluorogenic PCR assays (TaqMan) forrapid detection of Escherichia coli O157:H7 and other Shiga toxigenic E. coliMolecular and Cellular Probes, 1999
- Isolation ofMycobacterium paratuberculosisfrom Milk by Immunomagnetic SeparationApplied and Environmental Microbiology, 1998
- PCR-Based DNA Amplification and Presumptive Detection ofEscherichia coliO157:H7 with an Internal Fluorogenic Probe and the 5′ Nuclease (TaqMan) AssayApplied and Environmental Microbiology, 1998
- Use of commercial enzyme immunoassays and immunomagnetic separation systems for detecting Escherichia coli O157 in bovine fecal samplesApplied and Environmental Microbiology, 1997
- Detection of Escherichia coli O157:H7 by multiplex PCRJournal of Clinical Microbiology, 1995
- Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reactionJournal of Clinical Microbiology, 1989