Saturation kinetics of palmitate uptake in perfused skeletal muscle
- 25 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (2) , 327-329
Abstract
We investigated the kinetics of palmitate uptake in a physiological skeletal muscle preparation by using the isolated perfused rat hindquarter. When plotted against the unbound plasma palmitate concentration, palmitate uptake displayed a simple Michaelis‐Menten relation with a calculated Vmax and Km of 16.3 nmol · min−1 · g−1 and 0.06 μmol·l−1, respectively. These results show that, as in isolated cell systems, uptake of free fatty acids in perfused skeletal muscle follows saturation kinetics consistent with carrier‐mediated membrane transport of free fatty acids.Keywords
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