A pilot field study on Dengue vectors with locally manufactured inexpensive gravid female traps

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Abstract
High prevalence of Aedes aegypti and Ae. albopictus vectors and dengue incidence as well as the presence of the four dengue virus serotypes underscore the need for effective and simple tools to monitor dengue vectors in Sri Lanka. Field studies were therefore undertaken with inexpensive gravid female trap (GFT) fabricated from local materials in Jaffna city in northern Sri Lanka. The study was initiated in April 2022, and GFT-based mosquito collection was conducted from June 2022 to April 2023. Five blocks of 30 to 40 houses, were selected in areas of high dengue prevalence in Jaffna city. Thirty houses were selected within each block and provided with five traps to be placed indoor and outdoor in each house. Conventional larval indices were determined at the beginning, middle and end of the study. Nine rounds of GFT collections were performed to identify and quantify trapped mosquitoes, their parity, and infection with virus serotypes. A total of 2177 and 3069 Aedes were collected respectively in indoor and outdoor GFTs, during nine rounds of collections and identified at the species level where possible. A total of 679 and 433 Ae. aegypti and Ae. albopictus were collected in indoor traps and 377 and 1288 in outdoor traps respectively. Adult and larval indices decreased respectively by 50% and 100% by the end of the study. Parous mosquitoes comprised 93% (of 120) and 94% (of 288) of the collected Ae. aegypti and Ae. albopictus respectively. In 110 pools of female Ae. aegypti and Ae. albopictus screened for virus serotype by PCR, five pools of Ae. aegypti and six pools of Ae. albopictus from both indoor and outdoor collections were positive for DENV-2. GFTs fabricated with locally available and inexpensive materials described here can be effective for monitoring the prevalence of Aedes vector species, parity and dengue virus infection status. The larval indices suggest that the use of GFTs can contribute to reducing dengue transmission.
Funding Information
  • National Science Foundation of Sri Lanka (RG/2019/HS/02)
  • National Institutes of Health (5U01AI151788-02)

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