Properties of Alginate Lyases from Marine Bacteria
Authors:
- 1 April 1984
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 47 (4) , 699-703
Abstract
Alginate lyases (EC 4.2.2.3) from two marine bacteria were isolated and partially characterized. A cell-bound lyase from isolate A3 had a molecular weight of approximately 100,000 and cleaved mannuronate blocks, apparently in an exo manner. A lyase recovered from the culture medium of isolate W3 was soluble in saturated ammonium sulfate, cleaved guluronate blocks, apparently in an endo manner, and had a molecular weight of 35,000. The thiobarbiturate test and urea-polyacrylamide gel electrophoresis were used to determine substrate specificity and mode of substrate cleavage by the enzymes.Keywords
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