Expression of HarpinXoo in Transgenic Tobacco Induces Pathogen Defense in the Absence of Hypersensitive Cell Death
Authors:
- Jian-Ling PengJPJian-Ling Peng
- Zhi-Long BaoZBZhi-Long Bao
- Hai-Ying RenHRHai-Ying Ren
- Jin-Sheng WangJWJin-Sheng Wang
- Han-Song DongHDHan-Song Dong
Open Access
- 1 October 2004
- journal article
- Published by Scientific Societies in Phytopathology®
- Vol. 94 (10) , 1048-1055
Abstract
HarpinXoo, encoded by the hpaGXoo gene of Xanthomonas oryzae pv. oryzae, is a member of the harpin group of proteins that induce pathogen resistance and hypersensitive cell death (HCD) in plants. We elaborated whether both processes are correlated in hpaGXoo-expressing tobacco (HARTOB) plants, which produced harpinXoo intracellularly. Resistance to fungal, bacterial, and viral pathogens increased in HARTOB, in correlation with the expression of hpaGXoo, the gene NPR1 that regulates several resistance pathways, and defense genes GST1, Chia5, PR-1a, and PR-1b that are mediated by different signals. However, reactive oxygen intermediate burst, the expression of HCD marker genes hsr203 and hin1, and cell death did not occur spontaneously in HARTOB, though they did in untransformed and HARTOB plants treated exogenously with harpinXoo. Thus, the transgenic expression of harpinXoo confers nonspecific pathogen defense in the absence of HCD.Keywords
This publication has 50 references indexed in Scilit:
- Harpin Inactivates Mitochondria in Arabidopsis Suspension CellsMolecular Plant-Microbe Interactions®, 2004
- Inducers of Plant Systemic Acquired Resistance Regulate NPR1 Function through Redox ChangesCell, 2003
- Salicylic Acid and NIM1/NPR1-Independent Gene Induction by Incompatible Peronospora parasitica in ArabidopsisMolecular Plant-Microbe Interactions®, 2001
- Plant pathogens and integrated defence responses to infectionNature, 2001
- Harpin-Induced Hypersensitive Cell Death Is Associated with Altered Mitochondrial Functions in Tobacco CellsMolecular Plant-Microbe Interactions®, 2000
- Reduction of lesion growth rate of late blight plant disease in transgenic potato expressing harpin proteinScience China Life Sciences, 1999
- Analysis of Translation Elongation Factors from Wheat during Development and Following Heat ShockBiochemical and Biophysical Research Communications, 1998
- The Arabidopsis NPR1 Gene That Controls Systemic Acquired Resistance Encodes a Novel Protein Containing Ankyrin RepeatsCell, 1997
- Induction of systemic acquired resistance in cucumber by Pseudomonas syringae pv. syringae 61 HrpZPss proteinThe Plant Journal, 1996
- Harpin, Elicitor of the Hypersensitive Response Produced by the Plant Pathogen Erwinia amylovoraScience, 1992