Efficient Translation of Tobacco Mosaic Virus RNA and Rabbit Globin 9S RNA in a Cell-Free System from Commercial Wheat Germ
Authors:
- 1 August 1973
- journal article
- Published by National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (8) , 2330-2334
Abstract
Extracts prepared from commercially available wheat germ efficiently translate messenger RNAs from either viral or eukaryotic origin. The addition of tobacco mosaic virus RNA stimulated amino-acid incorporation more than 100-times and rabbit globin 9S RNA between 20- and 30-times. The in vitro product directed by globin 9S RNA comigrated precisely with authentic rabbit globin on sodium dodecyl sulfate-polyacrylamide gels. Furthermore, during high voltage ionophoresis two [ 35 S]methionine-labeled tryptic peptides synthesized in vitro comigrated in two dimensions with αT5 and βT5 tryptic peptides from authentic [ 35 S]methionine-labeled rabbit globin.Keywords
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