Modulation of an Outer Membrane Protease Contributes to the Virulence Defect of Shigella flexneri Strains Carrying a Mutation in the virK Locus
Open Access
- 1 February 2005
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 73 (2) , 1217-1220
Abstract
The Shigella actin assembly protein IcsA is removed from the bacterial surface by the protease IcsP. We show that decreased intracellular spreading of virK ::Tn 10 mutants is due in part to significant increases in IcsP and IcsP-mediated cleavage of IcsA and that IcsP expression is a critical determinant of Shigella virulence.Keywords
This publication has 30 references indexed in Scilit:
- Chromosomal and Plasmid-encoded Enzymes Are Required for Assembly of the R3-type Core Oligosaccharide in the Lipopolysaccharide of Escherichia coli O157:H7Journal of Biological Chemistry, 2004
- Simple and Reliable Method To Precipitate Proteins from Bacterial Culture SupernatantApplied and Environmental Microbiology, 2004
- Complete DNA Sequence and Analysis of the Large Virulence Plasmid of Shigella flexneriInfection and Immunity, 2001
- Siderophore-Mediated Iron Transport: Crystal Structure of FhuA with Bound LipopolysaccharideScience, 1998
- SopA, the outer membrane protease responsible for polar localization of IcsA in Shigella flexneriMolecular Microbiology, 1997
- The unrelated surface proteins ActA of Listeria monocytogenes and lcsA of Shigella flexneri are sufficient to confer actin‐based motility on Listeria innocua and Escherichia coli respectivelyMolecular Microbiology, 1995
- Identification and characterization of virK, a virulence‐associated large plasmid gene essential for intercellular spreading of Shigella flexneriMolecular Microbiology, 1992
- Construction of a physical map of the chromosome of Shigella flexneri 2a and the direct assignment of nine virulence‐associated loci identified by Tn5 insertionsMolecular Microbiology, 1991
- A genetic determinant required for continuous reinfection of adjacent cells on large plasmid in S. flexneri 2aCell, 1986
- Identification and nucleotide sequence of the activator gene of the externally induced phosphoglycerate transport system of Salmonella typhimuriumGene, 1986