Prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque

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Abstract
Six bacterial strains isolated from the human oral cavity, PPPA16, PPPA20T, PPPA24, PPPA31, EPPA6 and EPPA7, were characterized by determining phenotypic and biochemical features, cellular fatty acid profiles, menaquinone profiles and phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that the isolates represented the same species of the genusPrevotella. The strains were related toPrevotella dentaliswith about 89 % similarity. In addition, the isolates were related toPrevotellasp. oral clone IDR-CEC-0032, which is a representative of the numerically dominant cluster VI in carious dentine lesions [Nadkarniet al. (2004).J Clin Microbiol42, 5238–5244], with about 99 % similarity. The strains were obligately anaerobic, non-pigmenting, non-spore-forming, non-motile, Gram-negative rods. The isolates could be differentiated from otherPrevotellaspecies byd-mannitol,d-melezitose,d-sorbitol andd-trehalose fermentation in API 20A tests. The cellular fatty acid composition of strains PPPA16, PPPA20T, PPPA24, PPPA31, EPPA6 and EPPA7 was significantly different from that of otherPrevotellaspecies. Compared with otherPrevotellaspecies, only these six strains contained dimethyl acetals. The major menaquinones of the clinical isolates were MK-12 and MK-13, whereas the major menaquinones of otherPrevotellaspecies were MK-10 and MK-11. On the basis of these data, a novelPrevotellaspecies,Prevotella multisaccharivoraxsp. nov., is proposed, with PPPA20T(=JCM 12954T=DSM 17128T) as the type strain.

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