Effect of Inducible Expressed Human Cytomegalovirus Immediate Early 86 Protein on Cell Apoptosis
Authors:
- Ling LiLLLing Li
- Bin WangBWBin Wang
- Zhijun LiuZLZhijun Liu
- Haitao WangHWHaitao Wang
- Zhiyong YanZYZhiyong Yan
- Dongmeng QianDQDongmeng Qian
- Shouyi DingSDShouyi Ding
- Xuxia SongXSXuxia Song
- 23 June 2009
- journal article
- Published by Oxford University Press (OUP) in Bioscience, Biotechnology, and Biochemistry
- Vol. 73 (6) , 1268-1273
Abstract
Human cytomegalovirus is a common human pathogen that can cause life-threatening disease under certain conditions. During infection of host cells, the virus expresses regulatory proteins such as IE72 and IE86 that are important for viral propagation. IE86 plays a critical role in the modulation of viral replication as well as host cell cycle control and apoptosis. In this study, a Tet-On system was used to quantify the effect of IE86 on apoptosis and p53 expression. Our results indicate that IE86 inhibits tumor necrosis factor (TNF)-alpha induced apoptosis and that the anti-apoptotic activity of this viral protein correlates with its expression levels. In addition, IE86 did not alter the mRNA level of p53. The system developed should provide a method for functional analysis of human cytomegalovirus (HCMV) IE86 protein.Keywords
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