Rat embryonic stem cells produce fertile offspring through tetraploid complementation

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Abstract
Significance: Tetraploid complementation, through which an entire organism is produced from pluripotent donor cells, is taken as the most stringent test for pluripotency. However, it remains unclear whether embryonic stem cells (ESCs) of other species besides mice can pass this test. Our results demonstrated the capacity of rat ESCs to produce live rats via tetraploid complementation and how the capacity is lost during in vitro culture. This report demonstrates that ESCs of other species besides mice can pass the tetraploid complementation test for pluripotency. We believe this original work will facilitate the understanding of evolution and regulation of pluripotency across mammalian species.
Funding Information
  • National Natural Science Foundation of China (31422038, 31471395, 31501188)
  • Ministry of Science and Technology of the People's Republic of China (2015AA020307, 2014BAI02B01)
  • Ministry of Science and Technology of the People's Republic of China (2014CB964900, 2014CB964800)

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