Insertion of a New Gene of Viral Origin into Bone Marrow Cells of Mice
Authors:
- 30 May 1980
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 208 (4447) , 1033-1035
Abstract
DNA containing the herpes simplex virus thymidine kinase (HSVtk) gene was used to transform wild-type tk + mouse L cells to a tk ++ status in vitro using methotrexate as a selective agent. HSVtk DNA was also used to transform mouse bone marrow cells in vitro. Transformed marrow cells injected into irradiated and methotrexate-treated recipient mice gave rise to proliferating cells which in some cases dominated the marrow population and which contained HSVtk gene sequences.Keywords
This publication has 10 references indexed in Scilit:
- Gene transfer in intact animalsNature, 1980
- Rabbit β-globin mRNA production in mouse L cells transformed with cloned rabbit β-globin chromosomal DNANature, 1979
- Cloning of Herpes Simplex Type 1 DNA Fragments in a Bacteriophage Lambda VectorScience, 1979
- Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donorCell, 1978
- The transfer and stable integration of the HSV thymidine kinase gene into mouse cellsCell, 1978
- Biochemical transformation of mouse cells by fragments of herpes simplex virus DNACell, 1977
- Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells.Proceedings of the National Academy of Sciences, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Transfer of Thymidine Kinase to Thymidine Kinaseless L Cells by Infection with Ultraviolet-Irradiated Herpes Simplex VirusJournal of Virology, 1971
- A Direct Measurement of the Radiation Sensitivity of Normal Mouse Bone Marrow CellsRadiation Research, 1961