Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products
- 1 January 2014
- book chapter
- Published by Springer Nature
- Vol. 1373, 43-49
Abstract
Genetic manipulation of bacteria often requires the joining together of more than one DNA segment to form a hybrid DNA molecule. This can be accomplished by PCR followed by restriction endonuclease digestions and ligations. However, this approach can often become laborious and expensive. Here is described a well-established method for using primer design and PCR to obtain hybrid products for use in cloning vectors, mutagenesis protocols, and other applications.Keywords
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