Recombinant Interferon-γ Stimulates the Production of Human Tumor Necrosis Factor In Vitro

Authors:
  • PSPhilip Scuderi
    Philip Scuderi
  • KSKaren E. Sterling
    Karen E. Sterling
  • ARArthur B. Raitano
    Arthur B. Raitano
  • TGThomas M. Grogan
    Thomas M. Grogan
  • RRRichard A. Rippe
    Richard A. Rippe
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Abstract
Human peripheral blood monocytes in culture secrete tumor necrosis factor (TNF), which can be detected with a sensitive enzyme-linked immunosorbent assay system. When recombinant human interferon-γ (rIFN-γ) is added to cultured cells, TNF production is increased. rIFN-γ is also able to sustain the elevated production level of TNF over a 4-day period. Recombinant interferon-α (rlFN-α) was unable to stimulate increased TNF production. Unstimulated mononuclear phagocytes and rIFN-α-treated cells decreased secretion of TNF over a 4-day interval. The stimulatory effect of rIFN-γ was dose dependent and required both new RNA and protein synthesis and was independent of endotoxin in the tissue culture medium.

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