Cloning and expression of a novel mouse somatostatin receptor (SSTR2B)
Authors:
- Monika KoubaMKMonika Kouba
- Xiaomin WangXWXiaomin Wang
- Gudrun VogtGVGudrun Vogt
- Volker HölltVHVolker Höllt
- 26 October 1992
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 311 (3) , 290-294
Abstract
A mouse somatostatin (SS) receptor cDNA was cloned from neuroblastoma x glioma (NG108‐15) cells. The sequence is almost identical to that of the mouse SSTR2 receptor [(1992) Proc. Natl. Acad. Sci. USA 89, 251)] but lacks about 300 nucleotides between transmembrane domain VII and the C‐terminus. This spliced variant of SSTR2 (designated SSTR2B) encodes a protein which is 23 residues shorter than that predicted from the SSTR2 sequence, and differs in 15 amino acids at the C‐terminus. mRNA corresponding to SSTR2B occurs in mouse tissues in higher abundance than that of SSTR2. SSTR2B binds SS peptides with high affinity when expressed in mammalian cells.Keywords
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