Sequential affinity chromatography miniaturized within a “lab-on-valve” system
- 3 March 2005
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 130 (4) , 469-471
Abstract
An automatically renewable microcolumn, subjected to operation by programmable flow, is presented and for the first time used for separation and quantification of biomolecules on Sepharose Protein A beads, with absorbance measurement at 280 nm and a detection limit of 6 ng mouse IgG µL−1.Keywords
This publication has 2 references indexed in Scilit:
- Reversed-phase porous silica rods, an alternative approach to high-performance liquid chromatographic separation using the sequential injection chromatography techniqueJournal of Chromatography A, 2003
- Label dilution method: a novel tool for bioligand interaction studies using bead injection in the lab-on-valve formatThe Analyst, 2002