Paper chromatography of lipides

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Abstract
Summary: Procedures for qualitative and quantitative paper chromatography of lipides have been described in detail, together with some practical applications.With the monochain lipides it was found that one developing system serves universally. The long aliphatic chains essentially determine the chromatographic properties so that suitable conditions for chromatographing any lipide or lipide‐like entity are predictable. For the same reason total analysis of natural mixtures cannot be done on one paper chromatogram due to superpositions.For complete analysis of natural mixtures of fatty acids it is advisable at the present time to combine paper chromatography with some other separation method. Distillation supplements best but requires gram quantities of material while paper chromatography is applicable on gamma or milligram scale. Chain‐length analysis by paper chromatography and low‐temperature chromatography have been introduced and are promising methods to approach a completely micro method for analyzing complex mixtures.
Funding Information
  • National Institutes of Health
  • Hormel Foundation

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