A novel cholera toxin‐sensitive G‐protein (Gc) regulating receptor‐mediated phosphoinositide signalling in human pituitary clonal cells
Authors:
- William W.Y. LoWLWilliam W.Y. Lo
- John HughesJHJohn Hughes
- 17 August 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 220 (2) , 327-331
Abstract
Recent studies have implicated that a GTP‐binding protein (G‐protein) is involved in the coupling of both CCK‐8 and muscarinic cholinergic receptors to phosphoinositidase C (PIC) in the human embryonic pituitary cell line, Flow 9000. Pretreatment of these cells with cholera toxin, but not pertussis toxin, inhibited the stimulation of [3H]inositol phosphate production by CCK‐8 and acetylcholine. These inhibitory effects of cholera toxin could not be reproduced by treating the cells with the B‐subunit of cholera toxin or cAMP‐generating agents such as forskolin. These data suggest the presence of a novel Gc protein which is responsible for receptor‐PIC coupling in Flow 9000 cells.Keywords
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