The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization
Open Access
- 10 May 2023
- journal article
- review article
- Published by Frontiers Media SA in Frontiers in Microbiology
- Vol. 14
Abstract
This mini-review covers the use of nitrogen-15 in bacterial and fungal natural product discovery and biosynthetic characterization from 1970 to 2022. Nitrogen is an important element in a number of bioactive and structurally intriguing natural products including alkaloids, non-ribosomal peptides, and hybrid natural products. Nitrogen-15 can be detected at natural abundance utilizing two-dimensional nuclear magnetic resonance and mass spectrometry. Additionally, it is a stable isotope that can be added to growth media for both filamentous fungi and bacteria. With stable isotope feeding, additional two-dimensional nuclear magnetic resonance and mass spectrometry strategies have become available, and there is a growing trend to use nitrogen-15 stable isotope feeding for the biosynthetic characterization of natural products. This mini-review will catalog the use of these strategies, analyze the strengths and weaknesses of the different approaches, and suggest future directions for the use of nitrogen-15 in natural product discovery and biosynthetic characterization.Keywords
This publication has 49 references indexed in Scilit:
- Mass spectral molecular networking of living microbial coloniesProceedings of the National Academy of Sciences, 2012
- Microbial Strain Prioritization Using Metabolomics Tools for the Discovery of Natural ProductsAnalytical Chemistry, 2012
- Padanamides A and B, Highly Modified Linear Tetrapeptides Produced in Culture by a Streptomyces sp. Isolated from a Marine SedimentOrganic Letters, 2011
- Utilizing Long-Range1H-15N 2-D NMR Spectroscopy for Chemical Structure Elucidation and Confirmation2010
- NMR chemical shift data and ab initio shielding calculations: emerging tools for protein structure determinationChemical Society Reviews, 2009
- Interpretation of Tandem Mass Spectra Obtained from Cyclic Nonribosomal PeptidesAnalytical Chemistry, 2009
- Taumycins A and B, Two Bioactive Lipodepsipeptides from the Madagascar Sponge Fascaplysinopsis sp.Organic Letters, 2008
- Coibamide A, a Potent Antiproliferative Cyclic Depsipeptide from the Panamanian Marine Cyanobacterium Leptolyngbya sp.Journal of the American Chemical Society, 2008
- NMR nomenclature: Nuclear spin properties and conventions for chemical shifts (IUPAC recommendations 2001)Concepts in Magnetic Resonance, 2002
- 1H, 13C and 15N chemical shift referencing in biomolecular NMRJournal of Biomolecular NMR, 1995