Utilising the left-helical conformation of L-DNA for analysing different marker types on a single universal microarray platform

Authors:
  • RMRafael Martinez
    Rafael Martinez
  • AJAnette Jacob
    Anette Jacob
  • SRSteffen Rupp
    Steffen Rupp
  • JHJörg D. Hoheisel
    Jörg D. Hoheisel
  • SMStefan Matysiak
    Stefan Matysiak
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Abstract
L-DNA is the perfect mirror-image form of the naturally occurring d-conformation of DNA. Therefore, L-DNA duplexes have the same physical characteristics in terms of solubility, duplex stability and selectivity as D-DNA but form a left-helical double-helix. Because of its chiral difference, L-DNA does not bind to its naturally occurring D-DNA counterpart, however. We analysed some of the properties that are typical for L-DNA. For all the differences, L-DNA is chemically compatible with the D-form of DNA, so that chimeric molecules can be synthesized. We take advantage of the characteristics of L-DNA toward the establishment of a universal microarray that permits the analysis of different kinds of molecular diagnostic information in a single experiment on a single platform, in various combinations. Typical results for the measurement of transcript level variations, genotypic differences and DNA-protein interactions are presented. However, on the basis of the characteristic features of L-DNA, also other applications of this molecule type are discussed.

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