Aqueous solution structure of an intercalated actinomycin D-dATGCAT complex by two-dimensional and one-dimensional proton NMR

Authors:
  • SBStephen C. Brown
    Stephen C. Brown
  • KMKary Mullis
    Kary Mullis
  • CLCorey Levenson
    Corey Levenson
  • RSRichard H. Shafer
    Richard H. Shafer
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Abstract
Two-dimensional NOESY and COSY 1H NMR techniques have been employed to determine the conformation of the complex formed by actinomycin D and the hexanucleoside pentaphosphate dATGCAT. One-dimensional NOE experiments in H2O confirmed the intact nature of the oligonucleotide double helix within the complex. The drug chromophore was intercalated between the GC base pairs, with the pentapeptide lactones nestled in the minor groove. No significant conformational change of the pentapeptide lactones between bound and free drug was observed.