Identification of Type A Influenza Viruses from Wild Birds on the Delmarva Peninsula, 2007–10
Authors:
- 1 March 2017
- journal article
- Published by American Association of Avian Pathologists (AAAP) in Avian Diseases
- Vol. 61 (1) , 83-89
Abstract
SUMMARY Wild waterfowl and shorebirds in the Delaware-Maryland-Virginia (Delmarva) Peninsula region within the Atlantic Flyway were sampled as part of the Early Detection of Highly Pathogenic H5N1 Avian Influenza (AI) in Wild Migratory Birds program. The U.S. Department of Agriculture (USDA) and state wildlife agencies submitted 7858 samples for AI virus (AIV) testing by real-time reverse transcription PCR (rRT-PCR) to the University of Delaware Poultry Health System from April 2007 to March 2011. Virus isolation attempts were performed on samples with matrix gene cycle threshold (Ct) values ≤33.9. Using rRT-PCR, AIV was detected in 14% (1091/7857) of the samples. In species with sample sizes >100, American black duck (Anas rubripes; 28%), ruddy turnstone (Arenaria interpres; 27%), American green-winged teal (Anas crecca; 21%), semipalmated sandpiper (Calidris pusilla; 27%), greater snow goose (Chen caerulescens atlanticus; 12%), mallard (Anas platyrhynchos; 10%), and northern pintail (Anas acuta; 14%) sh... RESUMEN Identificación de virus de influenza tipo A en aves silvestres en la península de Delmarva, entre los años 2007-10. Se muestrearon aves acuáticas y playeras silvestres en la región de la Península formada por los estado de Delaware, Maryland y Virginia (Delmarva) dentro de la ruta migratoria del Atlántico, como parte del Programa para la Detección Temprana del Virus de la Influenza Aviar H5N1 de Alta Patogenicidad en Aves Migratorias Silvestres. El Departamento de Agricultura de los Estados Unidos (con las siglas en inglés USDA) y las agencias estatales de vida silvestre enviaron 7858 muestras para la detección del virus de la influenza aviar (AIV) por transcripción reversa y PCR en tiempo real (rRT-PCR) al Sistema de Salud Avícola de la Universidad de Delaware entre Abril del 2007 a Marzo del 2011. Los procedimientos de aislamiento viral se realizaron en muestras que mostraron valores de ciclo umbral (Ct) para el procedimiento dirigido contra la matriz (Ct) ≤33.9. Mediante rRT-PCR, se detectó al ...Keywords
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