THE RELATIVE BIOLOGICAL IMPORTANCE OF CYCLOBUTANE AND(6–4) PYRIMIDINE‐PYRIMIDONE DIMER PHOTOPRODUCTS IN HUMAN CELLS: EVIDENCE FROM A XERODERMA PIGMENTOSUM REVERTANT*
Authors:
- 1 July 1988
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 48 (1) , 41-49
Abstract
Abstract— The relative biological importance of(5–5,6–6) cyclobutane and(6–4) pyrimidine‐pyrimidone dimers in mammalian cells has been determined in a xeroderma pigmentosum (XP) revertant that repairs only the(6–4) photoproduct. Surprisingly, the majority of biological effects of UV light, including cell killing, sister chromatid exchange, mutagenesis, and inhibition and recovery of DNA and RNA synthesis, appear to be due to the(6–4) photoproduct and not to the cyclobutane dimer. Although the XP revertant repairs its own genomic DNA, it fails to repair damage in shuttle vectors, including the pZ189 mutational and chloramphenicol acetyl transferase transcriptional assay systems. The revertant is therefore a cell line for which shuttle vectors are inappropriate as models for genomic DNA repair, and may highlight a general weakness of shuttle vectors for the study of infrequent DNA lesions.Keywords
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