Liquid–liquid phase separation-assisted Raman microscopy for sensitive and label-free analysis of enzymatic reactions and protein–small molecule interactions

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Abstract
High-concentration PEG-based liquid–liquid phase separation concentrates biomolecules into droplets, enabling highly sensitive, label-free Raman analysis of enzymatic reactions and protein–small-molecule interactions in dilute, small-volume samples.
Funding Information
  • Japan Society for the Promotion of Science (JP20K21468)
  • Japan Society for the Promotion of Science (JP21H05261)
  • Japan Society for the Promotion of Science (JP22H02594)
  • Japan Society for the Promotion of Science (JP24K22008)
  • Core Research for Evolutional Science and Technology (JPMJCR2024)

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